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KMID : 0545119950050020096
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 2 p.96 ~ p.99
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Effect of Magnesium Sulfate on Product Inhibition of Sisomicin Production
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SHIN DHUL-SOO
HAN SANG-HUN
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Abstract
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Addition of 100mM MgSO_4 to a cell culture after 54 hours resulted in a 2.4-fold increase in the sisomicin titre compared to a control to which no MgSO_4 was added, and a considerable amount of intracellular sisomicin was liberated outside the cells. The occurrence of product inhibition in fermentation was confirmed by a reduction in net sisomicin production with increasing amounts of added sisomicin without addition of MgSO_4. All added sisomicin was bound to sisomicin-free cells in the absence of MgSO_4 whereas approximately 40% of added sisomicin was bound with the addition of 100mM MgSO_4. Under conditions of no enzmye synthesis, maintained by adding chloramphenicol to exclude product repression, sisomicin was produced in the presence of 100mM MgSO_4 but little sisomicin was produced in the absence of MgSO_4.
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